A simple method for purification of rat alpha-fetoprotein by affi-gel blue chromatography and disc electrophoresis.
نویسندگان
چکیده
منابع مشابه
Purification and immunochemical characterization of alpha-fetoprotein from rat fetal serum and liver.
Two alpha1-globulin bands of fetal serum with relative mobilities against bromophenol blue of 0.55 and 0.58 on 7% polyacrylamide gel electrophoresis reacted with a monospecific rabbit antiserum to alpha-fetoprotein (AFP). The former globulin band was clearly detected in the fetal liver supernatant. AFP was immunochemically purified from both the fetal serum and liver, and their electrophoretic ...
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BACKGROUND: Clostridium septicum has played a significant role as a causative agent of many acute fetal diseases in man and animals. Alpha- toxin is the main factor in the pathogenesis of C. septicum with hemolytic, necrotic and lethal activities. OBJECTIVES: The study was designed to evaluate alpha-toxin purification and antibody production rate against a local strain of C. septicum NH2 which ...
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عنوان ژورنال:
- Acta medica Okayama
دوره 35 6 شماره
صفحات -
تاریخ انتشار 1981